A MODIFIED PROCEDURE FOR LEAD STAINING OF THIN SECTIONS
نویسندگان
چکیده
منابع مشابه
A Modified Procedure for Lead Staining of Thin Sections
Meta l impregnat ion , or "s ta in ing ," of tissue sections for electron microscopy has become the accepted practice in recent years. Salts of metals of h igh atomic weight such as u ran ium, chromium, thor ium, lead, or tungsten, among many tested, have been found suitable (6, 1). "Lead hydroxide ," as prepar ted by Watson (6), is now widely used, hu t this solution is extremely unstable in a...
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Staining of thin sections results in greater contrast which facilitates focusing and the observation of fine structure. Lead hydroxide, as suggested by Watson (1), has become increasingly popular as a stain for electron microscopy. However, when lead hydroxide is exposed to the carbon dioxide of air, lead carbonate forms and is deposited on the sections as fine, needle-like crystals, large amor...
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A method of staining thin sections with lead hydroxide which enhances both resolution and contrast was described by M. L. Watson (1958) and is now undoubtedly in routine use in many laboratories. It is the purpose of this report to present a simplification of this method which eliminates the lengthy procedure of preparing the lead hydroxide reagent and the necessity of maintaining a COrfree atm...
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For more than 2 years, a double-staining of sections of OsO 4-fixed and Epon-embedded tissues with uranyl acetate followed by lead hydroxide has been routine technique in this laboratory. It was found to give substantially higher contrast, thus permitting the use of thinner sections. It facilitated focusing and routinely provided clearer pictures of cell fine structure than could be obtained wi...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1961
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.11.3.736